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| Cheret, Romuald; Hernandez Andres, Aránzazu; Delbarre Ladrat, Christine; De Lamballerie, Marie; Verrez-bagnis, Veronique. |
| Contrary to other preservation methods like thermal treatments, high pressure can destroy microorganisms without affecting the nutritional quality, color, or food texture. The firm texture of fish flesh is an important quality parameter. During the refrigerated storage, the tissue becomes softer and the muscle is deteriorated by different proteases. The aim of this study was to study the modification of the fish muscle proteins after high-pressure treatment during the refrigerated storage and to evaluate the effect of high-pressure treatment level on the post-mortem protein changes and enzyme activities. The calpain activity decreased with the high-pressure treatment and evolved differently during the refrigerated storage, depending on the level of... |
| Tipo: Text |
Palavras-chave: Cathepsins; Calpains; Sarcoplasmic proteins; Myofibrillar proteins; Post mortem denaturation; High pressure; Fish. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1708.pdf |
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| Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel. |
| The effects of a milli(m)-calpain isolated from the white muscle of sea bass (Dicentrarchus labrax L) and commercial cathepsins B, D and L, used in combination on the myofibrillar and sarcoplasmic proteins were examined. Protein digestion was first performed by the endogenous m-calpain, (luring 2 h before the addition of a mixture of cathepsins B, D and L and a further incubation up to 22 h. Calpain degraded a 27 kDa sarcoplasmic component as well as myosin heavy chain, a-actinin, desmin and a 32 kDa component from the myofibrillar fraction. A 97 kDa component and the assumed creatine kinase-aldolase doublet were degraded during the incubation of sarcoplasmic proteins with the cathepsin mixture while, among the myofibrillar proteins, myosin, actin,... |
| Tipo: Text |
Palavras-chave: Postmortem aging; Fish muscle; Proteolysis; Cathepsins; Neutral calcium dependent protease. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-1682.pdf |
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